Recombinant:Article Title: miR-708-5p is elevated in bipolar patients and can induce mood disorder-associated behavior in mice.
Article Snippet: .. Reagents and tools table Reagent/Resource Reference or Source Identifier or Catalog Number Experimental models C57BL/6 mice Janvier Laboratories (France) Mus musculus Primary hippocampal and cortical neurons from embryonic rats Janvier Laboratories (France) Rattus norvegicus Human PBMCs This manuscript Homo sapiens Recombinant DNA rAAV-hSyn-EGFP Addgene #114213 pmirGLO dual‐luciferase expression vector Promega E1330 Oligonucleotides and other sequence-based reagents miR-708-5p Taqman qPCR probe ThermoFisher 4427975 miR-16-5p Taqman qPCR probe ThermoFisher 4427975 miR-30a-5p Taqman qPCR probe ThermoFisher 4427975 miR-1248-5p Taqman qPCR probe ThermoFisher 4427975 miR-129-5p Taqman qPCR probe ThermoFisher 4427975 U6 snRNA Taqman qPCR probe ThermoFisher 4427975/001973 For primers used for cloning, see Appendix Table S2 This paper N/A Chemicals, Enzymes and other reagents TRIzol TM Reagent Thermo Fisher 15596026 Glycogen Ambion AM9510 Software Prism 10 GraphPad https://www.graphpad.com Reagent/Resource Reference or Source Identifier or Catalog Number Zen Microscopy Software Zeiss https://www.zeiss.com/ microscopy/en/products/ software/zeiss-zen.html edgeR Bioconductor http://bioconductor.org/ packages/edgeR/ Fiji ImageJ NIH https://imagej.net/ Inkscape Inkscape https://inkscape.org/ Image Lab 6 Bio-Rad http://www.bio-rad.com/enus/sku/1709690-image-labsoftware CFX Maestro Bio-Rad https://www.bio-rad.com/ench/product/cfx-maestrosoftware-for-cfx-real-time-pcrinstruments Endnote 21 Clarivate Analytics http://endnote.com Other Lipofectamine 2000 Thermo Fisher 11668019 LeukoLOCK Thermo Fisher AM1923 mirVanaTM Thermo Fisher AM1560 QuantiGene ViewRNA miRNA Cell Assay Kit Thermo Fisher QVCM0001 Phusion Hot Start Polymerase Thermo Fisher F549S Tissue-Tek O.C.T Compound Sakura Finetek Europe 4583 Aqua Poly Mount Chemie Brunschwig POL18606-20 Complete Protease Inhibitor Cocktail EDTAfree SigmaAldrich P8340 4x Laemmli Sample Buffer Bio-Rad 1610747 Mini-PROTEAN® TGXTM Precast Protein Gel Bio-Rad 4561094 ClarityTM Western ECL Substrate Bio-Rad 1705060 14 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on M arch 18, 2025 from IP 2400:1a00:b030:f63:a41d:1434:c4fe:173b. .. Reagent/Resource Reference or Source Identifier or Catalog Number TURBO DNase enzyme Thermo Fisher AM2238 iScript cDNA synthesis kit Bio-Rad 1708891 iTaq Universal SYBR Green Supermix Bio-Rad 1725121 TaqMan MicroRNA Reverse Transcription Kit Thermo Fisher 4366597 TaqMan Universal PCR Master Mix Thermo Fisher 4304437 MidiPrep Kit Macherey Nagel 740410 Passive lysis buffer (luciferase) Promega E194A Aqua-Poly/Mount Chemie Brunschwig POL18606-20 Trans-Blot Turbo system Bio-Rad 1704158 ClarityTM Western ECL Substrate Bio-Rad 1705060 DEPC Nuclease-free H2O Ambion AM9906
Expressing:Article Title: miR-708-5p is elevated in bipolar patients and can induce mood disorder-associated behavior in mice.
Article Snippet: .. Reagents and tools table Reagent/Resource Reference or Source Identifier or Catalog Number Experimental models C57BL/6 mice Janvier Laboratories (France) Mus musculus Primary hippocampal and cortical neurons from embryonic rats Janvier Laboratories (France) Rattus norvegicus Human PBMCs This manuscript Homo sapiens Recombinant DNA rAAV-hSyn-EGFP Addgene #114213 pmirGLO dual‐luciferase expression vector Promega E1330 Oligonucleotides and other sequence-based reagents miR-708-5p Taqman qPCR probe ThermoFisher 4427975 miR-16-5p Taqman qPCR probe ThermoFisher 4427975 miR-30a-5p Taqman qPCR probe ThermoFisher 4427975 miR-1248-5p Taqman qPCR probe ThermoFisher 4427975 miR-129-5p Taqman qPCR probe ThermoFisher 4427975 U6 snRNA Taqman qPCR probe ThermoFisher 4427975/001973 For primers used for cloning, see Appendix Table S2 This paper N/A Chemicals, Enzymes and other reagents TRIzol TM Reagent Thermo Fisher 15596026 Glycogen Ambion AM9510 Software Prism 10 GraphPad https://www.graphpad.com Reagent/Resource Reference or Source Identifier or Catalog Number Zen Microscopy Software Zeiss https://www.zeiss.com/ microscopy/en/products/ software/zeiss-zen.html edgeR Bioconductor http://bioconductor.org/ packages/edgeR/ Fiji ImageJ NIH https://imagej.net/ Inkscape Inkscape https://inkscape.org/ Image Lab 6 Bio-Rad http://www.bio-rad.com/enus/sku/1709690-image-labsoftware CFX Maestro Bio-Rad https://www.bio-rad.com/ench/product/cfx-maestrosoftware-for-cfx-real-time-pcrinstruments Endnote 21 Clarivate Analytics http://endnote.com Other Lipofectamine 2000 Thermo Fisher 11668019 LeukoLOCK Thermo Fisher AM1923 mirVanaTM Thermo Fisher AM1560 QuantiGene ViewRNA miRNA Cell Assay Kit Thermo Fisher QVCM0001 Phusion Hot Start Polymerase Thermo Fisher F549S Tissue-Tek O.C.T Compound Sakura Finetek Europe 4583 Aqua Poly Mount Chemie Brunschwig POL18606-20 Complete Protease Inhibitor Cocktail EDTAfree SigmaAldrich P8340 4x Laemmli Sample Buffer Bio-Rad 1610747 Mini-PROTEAN® TGXTM Precast Protein Gel Bio-Rad 4561094 ClarityTM Western ECL Substrate Bio-Rad 1705060 14 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on M arch 18, 2025 from IP 2400:1a00:b030:f63:a41d:1434:c4fe:173b. .. Reagent/Resource Reference or Source Identifier or Catalog Number TURBO DNase enzyme Thermo Fisher AM2238 iScript cDNA synthesis kit Bio-Rad 1708891 iTaq Universal SYBR Green Supermix Bio-Rad 1725121 TaqMan MicroRNA Reverse Transcription Kit Thermo Fisher 4366597 TaqMan Universal PCR Master Mix Thermo Fisher 4304437 MidiPrep Kit Macherey Nagel 740410 Passive lysis buffer (luciferase) Promega E194A Aqua-Poly/Mount Chemie Brunschwig POL18606-20 Trans-Blot Turbo system Bio-Rad 1704158 ClarityTM Western ECL Substrate Bio-Rad 1705060 DEPC Nuclease-free H2O Ambion AM9906
Plasmid Preparation:Article Title: miR-708-5p is elevated in bipolar patients and can induce mood disorder-associated behavior in mice.
Article Snippet: .. Reagents and tools table Reagent/Resource Reference or Source Identifier or Catalog Number Experimental models C57BL/6 mice Janvier Laboratories (France) Mus musculus Primary hippocampal and cortical neurons from embryonic rats Janvier Laboratories (France) Rattus norvegicus Human PBMCs This manuscript Homo sapiens Recombinant DNA rAAV-hSyn-EGFP Addgene #114213 pmirGLO dual‐luciferase expression vector Promega E1330 Oligonucleotides and other sequence-based reagents miR-708-5p Taqman qPCR probe ThermoFisher 4427975 miR-16-5p Taqman qPCR probe ThermoFisher 4427975 miR-30a-5p Taqman qPCR probe ThermoFisher 4427975 miR-1248-5p Taqman qPCR probe ThermoFisher 4427975 miR-129-5p Taqman qPCR probe ThermoFisher 4427975 U6 snRNA Taqman qPCR probe ThermoFisher 4427975/001973 For primers used for cloning, see Appendix Table S2 This paper N/A Chemicals, Enzymes and other reagents TRIzol TM Reagent Thermo Fisher 15596026 Glycogen Ambion AM9510 Software Prism 10 GraphPad https://www.graphpad.com Reagent/Resource Reference or Source Identifier or Catalog Number Zen Microscopy Software Zeiss https://www.zeiss.com/ microscopy/en/products/ software/zeiss-zen.html edgeR Bioconductor http://bioconductor.org/ packages/edgeR/ Fiji ImageJ NIH https://imagej.net/ Inkscape Inkscape https://inkscape.org/ Image Lab 6 Bio-Rad http://www.bio-rad.com/enus/sku/1709690-image-labsoftware CFX Maestro Bio-Rad https://www.bio-rad.com/ench/product/cfx-maestrosoftware-for-cfx-real-time-pcrinstruments Endnote 21 Clarivate Analytics http://endnote.com Other Lipofectamine 2000 Thermo Fisher 11668019 LeukoLOCK Thermo Fisher AM1923 mirVanaTM Thermo Fisher AM1560 QuantiGene ViewRNA miRNA Cell Assay Kit Thermo Fisher QVCM0001 Phusion Hot Start Polymerase Thermo Fisher F549S Tissue-Tek O.C.T Compound Sakura Finetek Europe 4583 Aqua Poly Mount Chemie Brunschwig POL18606-20 Complete Protease Inhibitor Cocktail EDTAfree SigmaAldrich P8340 4x Laemmli Sample Buffer Bio-Rad 1610747 Mini-PROTEAN® TGXTM Precast Protein Gel Bio-Rad 4561094 ClarityTM Western ECL Substrate Bio-Rad 1705060 14 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on M arch 18, 2025 from IP 2400:1a00:b030:f63:a41d:1434:c4fe:173b. .. Reagent/Resource Reference or Source Identifier or Catalog Number TURBO DNase enzyme Thermo Fisher AM2238 iScript cDNA synthesis kit Bio-Rad 1708891 iTaq Universal SYBR Green Supermix Bio-Rad 1725121 TaqMan MicroRNA Reverse Transcription Kit Thermo Fisher 4366597 TaqMan Universal PCR Master Mix Thermo Fisher 4304437 MidiPrep Kit Macherey Nagel 740410 Passive lysis buffer (luciferase) Promega E194A Aqua-Poly/Mount Chemie Brunschwig POL18606-20 Trans-Blot Turbo system Bio-Rad 1704158 ClarityTM Western ECL Substrate Bio-Rad 1705060 DEPC Nuclease-free H2O Ambion AM9906
Sequencing:Article Title: miR-708-5p is elevated in bipolar patients and can induce mood disorder-associated behavior in mice.
Article Snippet: .. Reagents and tools table Reagent/Resource Reference or Source Identifier or Catalog Number Experimental models C57BL/6 mice Janvier Laboratories (France) Mus musculus Primary hippocampal and cortical neurons from embryonic rats Janvier Laboratories (France) Rattus norvegicus Human PBMCs This manuscript Homo sapiens Recombinant DNA rAAV-hSyn-EGFP Addgene #114213 pmirGLO dual‐luciferase expression vector Promega E1330 Oligonucleotides and other sequence-based reagents miR-708-5p Taqman qPCR probe ThermoFisher 4427975 miR-16-5p Taqman qPCR probe ThermoFisher 4427975 miR-30a-5p Taqman qPCR probe ThermoFisher 4427975 miR-1248-5p Taqman qPCR probe ThermoFisher 4427975 miR-129-5p Taqman qPCR probe ThermoFisher 4427975 U6 snRNA Taqman qPCR probe ThermoFisher 4427975/001973 For primers used for cloning, see Appendix Table S2 This paper N/A Chemicals, Enzymes and other reagents TRIzol TM Reagent Thermo Fisher 15596026 Glycogen Ambion AM9510 Software Prism 10 GraphPad https://www.graphpad.com Reagent/Resource Reference or Source Identifier or Catalog Number Zen Microscopy Software Zeiss https://www.zeiss.com/ microscopy/en/products/ software/zeiss-zen.html edgeR Bioconductor http://bioconductor.org/ packages/edgeR/ Fiji ImageJ NIH https://imagej.net/ Inkscape Inkscape https://inkscape.org/ Image Lab 6 Bio-Rad http://www.bio-rad.com/enus/sku/1709690-image-labsoftware CFX Maestro Bio-Rad https://www.bio-rad.com/ench/product/cfx-maestrosoftware-for-cfx-real-time-pcrinstruments Endnote 21 Clarivate Analytics http://endnote.com Other Lipofectamine 2000 Thermo Fisher 11668019 LeukoLOCK Thermo Fisher AM1923 mirVanaTM Thermo Fisher AM1560 QuantiGene ViewRNA miRNA Cell Assay Kit Thermo Fisher QVCM0001 Phusion Hot Start Polymerase Thermo Fisher F549S Tissue-Tek O.C.T Compound Sakura Finetek Europe 4583 Aqua Poly Mount Chemie Brunschwig POL18606-20 Complete Protease Inhibitor Cocktail EDTAfree SigmaAldrich P8340 4x Laemmli Sample Buffer Bio-Rad 1610747 Mini-PROTEAN® TGXTM Precast Protein Gel Bio-Rad 4561094 ClarityTM Western ECL Substrate Bio-Rad 1705060 14 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on M arch 18, 2025 from IP 2400:1a00:b030:f63:a41d:1434:c4fe:173b. .. Reagent/Resource Reference or Source Identifier or Catalog Number TURBO DNase enzyme Thermo Fisher AM2238 iScript cDNA synthesis kit Bio-Rad 1708891 iTaq Universal SYBR Green Supermix Bio-Rad 1725121 TaqMan MicroRNA Reverse Transcription Kit Thermo Fisher 4366597 TaqMan Universal PCR Master Mix Thermo Fisher 4304437 MidiPrep Kit Macherey Nagel 740410 Passive lysis buffer (luciferase) Promega E194A Aqua-Poly/Mount Chemie Brunschwig POL18606-20 Trans-Blot Turbo system Bio-Rad 1704158 ClarityTM Western ECL Substrate Bio-Rad 1705060 DEPC Nuclease-free H2O Ambion AM9906
Real-time Polymerase Chain Reaction:Article Title: miR-708-5p is elevated in bipolar patients and can induce mood disorder-associated behavior in mice.
Article Snippet: .. Reagents and tools table Reagent/Resource Reference or Source Identifier or Catalog Number Experimental models C57BL/6 mice Janvier Laboratories (France) Mus musculus Primary hippocampal and cortical neurons from embryonic rats Janvier Laboratories (France) Rattus norvegicus Human PBMCs This manuscript Homo sapiens Recombinant DNA rAAV-hSyn-EGFP Addgene #114213 pmirGLO dual‐luciferase expression vector Promega E1330 Oligonucleotides and other sequence-based reagents miR-708-5p Taqman qPCR probe ThermoFisher 4427975 miR-16-5p Taqman qPCR probe ThermoFisher 4427975 miR-30a-5p Taqman qPCR probe ThermoFisher 4427975 miR-1248-5p Taqman qPCR probe ThermoFisher 4427975 miR-129-5p Taqman qPCR probe ThermoFisher 4427975 U6 snRNA Taqman qPCR probe ThermoFisher 4427975/001973 For primers used for cloning, see Appendix Table S2 This paper N/A Chemicals, Enzymes and other reagents TRIzol TM Reagent Thermo Fisher 15596026 Glycogen Ambion AM9510 Software Prism 10 GraphPad https://www.graphpad.com Reagent/Resource Reference or Source Identifier or Catalog Number Zen Microscopy Software Zeiss https://www.zeiss.com/ microscopy/en/products/ software/zeiss-zen.html edgeR Bioconductor http://bioconductor.org/ packages/edgeR/ Fiji ImageJ NIH https://imagej.net/ Inkscape Inkscape https://inkscape.org/ Image Lab 6 Bio-Rad http://www.bio-rad.com/enus/sku/1709690-image-labsoftware CFX Maestro Bio-Rad https://www.bio-rad.com/ench/product/cfx-maestrosoftware-for-cfx-real-time-pcrinstruments Endnote 21 Clarivate Analytics http://endnote.com Other Lipofectamine 2000 Thermo Fisher 11668019 LeukoLOCK Thermo Fisher AM1923 mirVanaTM Thermo Fisher AM1560 QuantiGene ViewRNA miRNA Cell Assay Kit Thermo Fisher QVCM0001 Phusion Hot Start Polymerase Thermo Fisher F549S Tissue-Tek O.C.T Compound Sakura Finetek Europe 4583 Aqua Poly Mount Chemie Brunschwig POL18606-20 Complete Protease Inhibitor Cocktail EDTAfree SigmaAldrich P8340 4x Laemmli Sample Buffer Bio-Rad 1610747 Mini-PROTEAN® TGXTM Precast Protein Gel Bio-Rad 4561094 ClarityTM Western ECL Substrate Bio-Rad 1705060 14 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on M arch 18, 2025 from IP 2400:1a00:b030:f63:a41d:1434:c4fe:173b. .. Reagent/Resource Reference or Source Identifier or Catalog Number TURBO DNase enzyme Thermo Fisher AM2238 iScript cDNA synthesis kit Bio-Rad 1708891 iTaq Universal SYBR Green Supermix Bio-Rad 1725121 TaqMan MicroRNA Reverse Transcription Kit Thermo Fisher 4366597 TaqMan Universal PCR Master Mix Thermo Fisher 4304437 MidiPrep Kit Macherey Nagel 740410 Passive lysis buffer (luciferase) Promega E194A Aqua-Poly/Mount Chemie Brunschwig POL18606-20 Trans-Blot Turbo system Bio-Rad 1704158 ClarityTM Western ECL Substrate Bio-Rad 1705060 DEPC Nuclease-free H2O Ambion AM9906
Cloning:Article Title: miR-708-5p is elevated in bipolar patients and can induce mood disorder-associated behavior in mice.
Article Snippet: .. Reagents and tools table Reagent/Resource Reference or Source Identifier or Catalog Number Experimental models C57BL/6 mice Janvier Laboratories (France) Mus musculus Primary hippocampal and cortical neurons from embryonic rats Janvier Laboratories (France) Rattus norvegicus Human PBMCs This manuscript Homo sapiens Recombinant DNA rAAV-hSyn-EGFP Addgene #114213 pmirGLO dual‐luciferase expression vector Promega E1330 Oligonucleotides and other sequence-based reagents miR-708-5p Taqman qPCR probe ThermoFisher 4427975 miR-16-5p Taqman qPCR probe ThermoFisher 4427975 miR-30a-5p Taqman qPCR probe ThermoFisher 4427975 miR-1248-5p Taqman qPCR probe ThermoFisher 4427975 miR-129-5p Taqman qPCR probe ThermoFisher 4427975 U6 snRNA Taqman qPCR probe ThermoFisher 4427975/001973 For primers used for cloning, see Appendix Table S2 This paper N/A Chemicals, Enzymes and other reagents TRIzol TM Reagent Thermo Fisher 15596026 Glycogen Ambion AM9510 Software Prism 10 GraphPad https://www.graphpad.com Reagent/Resource Reference or Source Identifier or Catalog Number Zen Microscopy Software Zeiss https://www.zeiss.com/ microscopy/en/products/ software/zeiss-zen.html edgeR Bioconductor http://bioconductor.org/ packages/edgeR/ Fiji ImageJ NIH https://imagej.net/ Inkscape Inkscape https://inkscape.org/ Image Lab 6 Bio-Rad http://www.bio-rad.com/enus/sku/1709690-image-labsoftware CFX Maestro Bio-Rad https://www.bio-rad.com/ench/product/cfx-maestrosoftware-for-cfx-real-time-pcrinstruments Endnote 21 Clarivate Analytics http://endnote.com Other Lipofectamine 2000 Thermo Fisher 11668019 LeukoLOCK Thermo Fisher AM1923 mirVanaTM Thermo Fisher AM1560 QuantiGene ViewRNA miRNA Cell Assay Kit Thermo Fisher QVCM0001 Phusion Hot Start Polymerase Thermo Fisher F549S Tissue-Tek O.C.T Compound Sakura Finetek Europe 4583 Aqua Poly Mount Chemie Brunschwig POL18606-20 Complete Protease Inhibitor Cocktail EDTAfree SigmaAldrich P8340 4x Laemmli Sample Buffer Bio-Rad 1610747 Mini-PROTEAN® TGXTM Precast Protein Gel Bio-Rad 4561094 ClarityTM Western ECL Substrate Bio-Rad 1705060 14 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on M arch 18, 2025 from IP 2400:1a00:b030:f63:a41d:1434:c4fe:173b. .. Reagent/Resource Reference or Source Identifier or Catalog Number TURBO DNase enzyme Thermo Fisher AM2238 iScript cDNA synthesis kit Bio-Rad 1708891 iTaq Universal SYBR Green Supermix Bio-Rad 1725121 TaqMan MicroRNA Reverse Transcription Kit Thermo Fisher 4366597 TaqMan Universal PCR Master Mix Thermo Fisher 4304437 MidiPrep Kit Macherey Nagel 740410 Passive lysis buffer (luciferase) Promega E194A Aqua-Poly/Mount Chemie Brunschwig POL18606-20 Trans-Blot Turbo system Bio-Rad 1704158 ClarityTM Western ECL Substrate Bio-Rad 1705060 DEPC Nuclease-free H2O Ambion AM9906
Software:Article Title: miR-708-5p is elevated in bipolar patients and can induce mood disorder-associated behavior in mice.
Article Snippet: .. Reagents and tools table Reagent/Resource Reference or Source Identifier or Catalog Number Experimental models C57BL/6 mice Janvier Laboratories (France) Mus musculus Primary hippocampal and cortical neurons from embryonic rats Janvier Laboratories (France) Rattus norvegicus Human PBMCs This manuscript Homo sapiens Recombinant DNA rAAV-hSyn-EGFP Addgene #114213 pmirGLO dual‐luciferase expression vector Promega E1330 Oligonucleotides and other sequence-based reagents miR-708-5p Taqman qPCR probe ThermoFisher 4427975 miR-16-5p Taqman qPCR probe ThermoFisher 4427975 miR-30a-5p Taqman qPCR probe ThermoFisher 4427975 miR-1248-5p Taqman qPCR probe ThermoFisher 4427975 miR-129-5p Taqman qPCR probe ThermoFisher 4427975 U6 snRNA Taqman qPCR probe ThermoFisher 4427975/001973 For primers used for cloning, see Appendix Table S2 This paper N/A Chemicals, Enzymes and other reagents TRIzol TM Reagent Thermo Fisher 15596026 Glycogen Ambion AM9510 Software Prism 10 GraphPad https://www.graphpad.com Reagent/Resource Reference or Source Identifier or Catalog Number Zen Microscopy Software Zeiss https://www.zeiss.com/ microscopy/en/products/ software/zeiss-zen.html edgeR Bioconductor http://bioconductor.org/ packages/edgeR/ Fiji ImageJ NIH https://imagej.net/ Inkscape Inkscape https://inkscape.org/ Image Lab 6 Bio-Rad http://www.bio-rad.com/enus/sku/1709690-image-labsoftware CFX Maestro Bio-Rad https://www.bio-rad.com/ench/product/cfx-maestrosoftware-for-cfx-real-time-pcrinstruments Endnote 21 Clarivate Analytics http://endnote.com Other Lipofectamine 2000 Thermo Fisher 11668019 LeukoLOCK Thermo Fisher AM1923 mirVanaTM Thermo Fisher AM1560 QuantiGene ViewRNA miRNA Cell Assay Kit Thermo Fisher QVCM0001 Phusion Hot Start Polymerase Thermo Fisher F549S Tissue-Tek O.C.T Compound Sakura Finetek Europe 4583 Aqua Poly Mount Chemie Brunschwig POL18606-20 Complete Protease Inhibitor Cocktail EDTAfree SigmaAldrich P8340 4x Laemmli Sample Buffer Bio-Rad 1610747 Mini-PROTEAN® TGXTM Precast Protein Gel Bio-Rad 4561094 ClarityTM Western ECL Substrate Bio-Rad 1705060 14 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on M arch 18, 2025 from IP 2400:1a00:b030:f63:a41d:1434:c4fe:173b. .. Reagent/Resource Reference or Source Identifier or Catalog Number TURBO DNase enzyme Thermo Fisher AM2238 iScript cDNA synthesis kit Bio-Rad 1708891 iTaq Universal SYBR Green Supermix Bio-Rad 1725121 TaqMan MicroRNA Reverse Transcription Kit Thermo Fisher 4366597 TaqMan Universal PCR Master Mix Thermo Fisher 4304437 MidiPrep Kit Macherey Nagel 740410 Passive lysis buffer (luciferase) Promega E194A Aqua-Poly/Mount Chemie Brunschwig POL18606-20 Trans-Blot Turbo system Bio-Rad 1704158 ClarityTM Western ECL Substrate Bio-Rad 1705060 DEPC Nuclease-free H2O Ambion AM9906
Microscopy:Article Title: miR-708-5p is elevated in bipolar patients and can induce mood disorder-associated behavior in mice.
Article Snippet: .. Reagents and tools table Reagent/Resource Reference or Source Identifier or Catalog Number Experimental models C57BL/6 mice Janvier Laboratories (France) Mus musculus Primary hippocampal and cortical neurons from embryonic rats Janvier Laboratories (France) Rattus norvegicus Human PBMCs This manuscript Homo sapiens Recombinant DNA rAAV-hSyn-EGFP Addgene #114213 pmirGLO dual‐luciferase expression vector Promega E1330 Oligonucleotides and other sequence-based reagents miR-708-5p Taqman qPCR probe ThermoFisher 4427975 miR-16-5p Taqman qPCR probe ThermoFisher 4427975 miR-30a-5p Taqman qPCR probe ThermoFisher 4427975 miR-1248-5p Taqman qPCR probe ThermoFisher 4427975 miR-129-5p Taqman qPCR probe ThermoFisher 4427975 U6 snRNA Taqman qPCR probe ThermoFisher 4427975/001973 For primers used for cloning, see Appendix Table S2 This paper N/A Chemicals, Enzymes and other reagents TRIzol TM Reagent Thermo Fisher 15596026 Glycogen Ambion AM9510 Software Prism 10 GraphPad https://www.graphpad.com Reagent/Resource Reference or Source Identifier or Catalog Number Zen Microscopy Software Zeiss https://www.zeiss.com/ microscopy/en/products/ software/zeiss-zen.html edgeR Bioconductor http://bioconductor.org/ packages/edgeR/ Fiji ImageJ NIH https://imagej.net/ Inkscape Inkscape https://inkscape.org/ Image Lab 6 Bio-Rad http://www.bio-rad.com/enus/sku/1709690-image-labsoftware CFX Maestro Bio-Rad https://www.bio-rad.com/ench/product/cfx-maestrosoftware-for-cfx-real-time-pcrinstruments Endnote 21 Clarivate Analytics http://endnote.com Other Lipofectamine 2000 Thermo Fisher 11668019 LeukoLOCK Thermo Fisher AM1923 mirVanaTM Thermo Fisher AM1560 QuantiGene ViewRNA miRNA Cell Assay Kit Thermo Fisher QVCM0001 Phusion Hot Start Polymerase Thermo Fisher F549S Tissue-Tek O.C.T Compound Sakura Finetek Europe 4583 Aqua Poly Mount Chemie Brunschwig POL18606-20 Complete Protease Inhibitor Cocktail EDTAfree SigmaAldrich P8340 4x Laemmli Sample Buffer Bio-Rad 1610747 Mini-PROTEAN® TGXTM Precast Protein Gel Bio-Rad 4561094 ClarityTM Western ECL Substrate Bio-Rad 1705060 14 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on M arch 18, 2025 from IP 2400:1a00:b030:f63:a41d:1434:c4fe:173b. .. Reagent/Resource Reference or Source Identifier or Catalog Number TURBO DNase enzyme Thermo Fisher AM2238 iScript cDNA synthesis kit Bio-Rad 1708891 iTaq Universal SYBR Green Supermix Bio-Rad 1725121 TaqMan MicroRNA Reverse Transcription Kit Thermo Fisher 4366597 TaqMan Universal PCR Master Mix Thermo Fisher 4304437 MidiPrep Kit Macherey Nagel 740410 Passive lysis buffer (luciferase) Promega E194A Aqua-Poly/Mount Chemie Brunschwig POL18606-20 Trans-Blot Turbo system Bio-Rad 1704158 ClarityTM Western ECL Substrate Bio-Rad 1705060 DEPC Nuclease-free H2O Ambion AM9906
Protease Inhibitor:Article Title: miR-708-5p is elevated in bipolar patients and can induce mood disorder-associated behavior in mice.
Article Snippet: .. Reagents and tools table Reagent/Resource Reference or Source Identifier or Catalog Number Experimental models C57BL/6 mice Janvier Laboratories (France) Mus musculus Primary hippocampal and cortical neurons from embryonic rats Janvier Laboratories (France) Rattus norvegicus Human PBMCs This manuscript Homo sapiens Recombinant DNA rAAV-hSyn-EGFP Addgene #114213 pmirGLO dual‐luciferase expression vector Promega E1330 Oligonucleotides and other sequence-based reagents miR-708-5p Taqman qPCR probe ThermoFisher 4427975 miR-16-5p Taqman qPCR probe ThermoFisher 4427975 miR-30a-5p Taqman qPCR probe ThermoFisher 4427975 miR-1248-5p Taqman qPCR probe ThermoFisher 4427975 miR-129-5p Taqman qPCR probe ThermoFisher 4427975 U6 snRNA Taqman qPCR probe ThermoFisher 4427975/001973 For primers used for cloning, see Appendix Table S2 This paper N/A Chemicals, Enzymes and other reagents TRIzol TM Reagent Thermo Fisher 15596026 Glycogen Ambion AM9510 Software Prism 10 GraphPad https://www.graphpad.com Reagent/Resource Reference or Source Identifier or Catalog Number Zen Microscopy Software Zeiss https://www.zeiss.com/ microscopy/en/products/ software/zeiss-zen.html edgeR Bioconductor http://bioconductor.org/ packages/edgeR/ Fiji ImageJ NIH https://imagej.net/ Inkscape Inkscape https://inkscape.org/ Image Lab 6 Bio-Rad http://www.bio-rad.com/enus/sku/1709690-image-labsoftware CFX Maestro Bio-Rad https://www.bio-rad.com/ench/product/cfx-maestrosoftware-for-cfx-real-time-pcrinstruments Endnote 21 Clarivate Analytics http://endnote.com Other Lipofectamine 2000 Thermo Fisher 11668019 LeukoLOCK Thermo Fisher AM1923 mirVanaTM Thermo Fisher AM1560 QuantiGene ViewRNA miRNA Cell Assay Kit Thermo Fisher QVCM0001 Phusion Hot Start Polymerase Thermo Fisher F549S Tissue-Tek O.C.T Compound Sakura Finetek Europe 4583 Aqua Poly Mount Chemie Brunschwig POL18606-20 Complete Protease Inhibitor Cocktail EDTAfree SigmaAldrich P8340 4x Laemmli Sample Buffer Bio-Rad 1610747 Mini-PROTEAN® TGXTM Precast Protein Gel Bio-Rad 4561094 ClarityTM Western ECL Substrate Bio-Rad 1705060 14 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on M arch 18, 2025 from IP 2400:1a00:b030:f63:a41d:1434:c4fe:173b. .. Reagent/Resource Reference or Source Identifier or Catalog Number TURBO DNase enzyme Thermo Fisher AM2238 iScript cDNA synthesis kit Bio-Rad 1708891 iTaq Universal SYBR Green Supermix Bio-Rad 1725121 TaqMan MicroRNA Reverse Transcription Kit Thermo Fisher 4366597 TaqMan Universal PCR Master Mix Thermo Fisher 4304437 MidiPrep Kit Macherey Nagel 740410 Passive lysis buffer (luciferase) Promega E194A Aqua-Poly/Mount Chemie Brunschwig POL18606-20 Trans-Blot Turbo system Bio-Rad 1704158 ClarityTM Western ECL Substrate Bio-Rad 1705060 DEPC Nuclease-free H2O Ambion AM9906
Western Blot:Article Title: miR-708-5p is elevated in bipolar patients and can induce mood disorder-associated behavior in mice.
Article Snippet: .. Reagents and tools table Reagent/Resource Reference or Source Identifier or Catalog Number Experimental models C57BL/6 mice Janvier Laboratories (France) Mus musculus Primary hippocampal and cortical neurons from embryonic rats Janvier Laboratories (France) Rattus norvegicus Human PBMCs This manuscript Homo sapiens Recombinant DNA rAAV-hSyn-EGFP Addgene #114213 pmirGLO dual‐luciferase expression vector Promega E1330 Oligonucleotides and other sequence-based reagents miR-708-5p Taqman qPCR probe ThermoFisher 4427975 miR-16-5p Taqman qPCR probe ThermoFisher 4427975 miR-30a-5p Taqman qPCR probe ThermoFisher 4427975 miR-1248-5p Taqman qPCR probe ThermoFisher 4427975 miR-129-5p Taqman qPCR probe ThermoFisher 4427975 U6 snRNA Taqman qPCR probe ThermoFisher 4427975/001973 For primers used for cloning, see Appendix Table S2 This paper N/A Chemicals, Enzymes and other reagents TRIzol TM Reagent Thermo Fisher 15596026 Glycogen Ambion AM9510 Software Prism 10 GraphPad https://www.graphpad.com Reagent/Resource Reference or Source Identifier or Catalog Number Zen Microscopy Software Zeiss https://www.zeiss.com/ microscopy/en/products/ software/zeiss-zen.html edgeR Bioconductor http://bioconductor.org/ packages/edgeR/ Fiji ImageJ NIH https://imagej.net/ Inkscape Inkscape https://inkscape.org/ Image Lab 6 Bio-Rad http://www.bio-rad.com/enus/sku/1709690-image-labsoftware CFX Maestro Bio-Rad https://www.bio-rad.com/ench/product/cfx-maestrosoftware-for-cfx-real-time-pcrinstruments Endnote 21 Clarivate Analytics http://endnote.com Other Lipofectamine 2000 Thermo Fisher 11668019 LeukoLOCK Thermo Fisher AM1923 mirVanaTM Thermo Fisher AM1560 QuantiGene ViewRNA miRNA Cell Assay Kit Thermo Fisher QVCM0001 Phusion Hot Start Polymerase Thermo Fisher F549S Tissue-Tek O.C.T Compound Sakura Finetek Europe 4583 Aqua Poly Mount Chemie Brunschwig POL18606-20 Complete Protease Inhibitor Cocktail EDTAfree SigmaAldrich P8340 4x Laemmli Sample Buffer Bio-Rad 1610747 Mini-PROTEAN® TGXTM Precast Protein Gel Bio-Rad 4561094 ClarityTM Western ECL Substrate Bio-Rad 1705060 14 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on M arch 18, 2025 from IP 2400:1a00:b030:f63:a41d:1434:c4fe:173b. .. Reagent/Resource Reference or Source Identifier or Catalog Number TURBO DNase enzyme Thermo Fisher AM2238 iScript cDNA synthesis kit Bio-Rad 1708891 iTaq Universal SYBR Green Supermix Bio-Rad 1725121 TaqMan MicroRNA Reverse Transcription Kit Thermo Fisher 4366597 TaqMan Universal PCR Master Mix Thermo Fisher 4304437 MidiPrep Kit Macherey Nagel 740410 Passive lysis buffer (luciferase) Promega E194A Aqua-Poly/Mount Chemie Brunschwig POL18606-20 Trans-Blot Turbo system Bio-Rad 1704158 ClarityTM Western ECL Substrate Bio-Rad 1705060 DEPC Nuclease-free H2O Ambion AM9906
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